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BT603: Biosensors
Assignment No. 1
Spring 2020
Due Date: 28 May 2020
Audio Introduction:
“Please prepare an audio introduction file through any available device. Preferably Sound recorder of the Windows”
General instructions
• Total time of audio introduction will be 5 minutes.
o Course name and code (30 sec) (Very Important)
o Your Introduction (2 minutes)
o Your academic Background (1 minute)
o Views about course (1 minute)
o Expectations about the course (30 sec)
• This assignment carries 10 marks.
• Audio Introduction file should be uploaded on LMS not later than 28 May 2020DO NOT send the assignment thorough email. Assignments submitted through email will result in no marks.
Instructions for audio recording through Sound recorder of the windows
• Open Windows Search
• Write “Sound recorder” in the search bar and click enter
• Select the program “Sound recorder” Open it and start recording.
• Save the file and send it through the LMS.
Outcomes:
This assignment will help you to develop presentation skills and strengthen you to present yourself in front of audience. -
Total Marks 5
Starting Date Monday, August 19, 2019
Closing Date Tuesday, August 20, 2019
Status Open
Question Title Process Organism selection
Question Description
Which of the following is preferable method for accurate identification of bacteria?Phenotypic method
Genotypic method
Support your answer with five well-versed statements.Important Instructions:
Your discussion must be based on logical facts.
Your comments on the topic should not exceed 200-250 words.
The GDB will remain open for 2 working days/ 48 hours.
Do not copy or exchange your answer with other students. Two identical / copied comments will be marked Zero (0) and may damage your grade in the course.
Obnoxious or ignoble answer should be strictly avoided.
Questions / queries related to the content of the GDB, which may be posted by the students on MDB or via e-mail, will not be replied till the due date of GDB is over.
Ø For Detailed Instructions please see the GDB Announcement
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Assignment No. 2
BT603 – Fermentation Technology
Fall 2019Total Marks: 10
Due date: 22-01-2020
Instructions:
• Make sure that you upload the solution file before due date. No assignment will be accepted through e-mail after the due date.
Formatting guidelines
• Use the font style “Times New Roman” and font size “12”.
• Compose your document in MS-Word, any file created in any format will not be accepted and marked zero.
• Use black and blue font colors only.
Solution guidelines
• To solve this assignment, you should have good command over lectures 38-51.
• This is not a group assignment, it is an individual assignment so be careful and avoid copying others’ work
• Give the answer according to question only and avoid irrelevant details.Please note that your assignment will not be graded if:
• It is submitted after due date
• The file you uploaded does not open
• The file you uploaded is copied from someone else
• It is in some format other than .doc
• Cheating or copying of assignment is strictly prohibited. The cheated or copied assignment will be marked ‘Zero’.Question No. 1
How foaming is produced in the fermentation media? Explain at least two different methods used to control foaming. (1+4)Question No. 2
What is meant by aeration? Describe any three methods of providing aeration to the fermentation process. (2+3)---------------------------------------------------GOOD LUCK----------------------------------------------
UNSOLVED BT603 GDB 1 Solution and Discussion
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Total Marks 5
Starting Date Monday, August 19, 2019
Closing Date Tuesday, August 20, 2019
Status Open
Question Title Process Organism selection
Question Description
Which of the following is preferable method for accurate identification of bacteria?Phenotypic method
Genotypic method
Support your answer with five well-versed statements.Important Instructions:
-
Your discussion must be based on logical facts.
-
Your comments on the topic should not exceed 200-250 words.
-
The GDB will remain open for 2 working days/ 48 hours.
-
Do not copy or exchange your answer with other students. Two identical / copied comments will be marked Zero (0) and may damage your grade in the course.
-
Obnoxious or ignoble answer should be strictly avoided.
-
Questions / queries related to the content of the GDB, which may be posted by the students on MDB or via e-mail, will not be replied till the due date of GDB is over.
Ø For Detailed Instructions please see the GDB Announcement
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@zareen said in BT603 GDB 1 Solution and Discussion:
Which of the following is preferable method for the long term storage of Microoraganisms?
Microbial resource centers and preservation research … Lyophilization is the preferred long-term preservation method in most MRCs due to the …
Lyophilization is the preferred long-term preservation method in most MRCs due to the low cost of maintenance and ease of transportation of lyophilized cultures. Lyophilization gives satisfactory results for the preservation of many bacteria, yeast and sporulating fungi, but does not adequately preserve non-sporulating fungi (vegetative hyphae), some species of yeast (Lipomyces, Leucosporidium, Brettanomyces, Dekkera, Bulleera, Sporobolomyces) and certain bacteria [Aquaspirillum serpens, Clostridium botulinum, Helicobacter pylori; Smith et al., 2008)]. Lyophilization exerts stress on the cells during vacuum desiccation, and cells raised under stress may respond better to lyophilization (Morgan et al., 2006; Smith et al., 2008). For instance, a culture at stationary phase and low pH condition survived better during lyophilization than did cells in log phase grown at circumneutral pH (Palmfeldt & Hahn-Hagerdal, 2000; Corcoran et al., 2004). However, the above-mentioned generalization is not true for all groups of bacteria (Boumahdi et al., 1999). In general, a suspension medium with 1 × 108 cell mL−1 or more gives a better recovery, whereas glass-forming cryoprotectants are preferred over a eutectic crystallization salt (palmfeldt et al., 2003; Morgan et al., 2006). Although techniques of lyophilization are well established, optimization of lyoprotectants and suspension media are still necessary for certain microorganisms (Crowe et al., 1998; Miyamoto-Shinohara et al., 2000; Carvalho et al., 2003; Gomez Zavaglia et al., 2003; Streeter, 2003; Berner & Viernstein, 2006; Tindall, 2007; Siaterlis et al., 2009). An ideal suspension medium for lyophilization should contain lyoprotectants and matrix materials or excipients. The use of stationary phase cultures, borosilicate ampules, a 1–2% final moisture content of the lyophilized specimen, and storage at 4 °C in the dark are recommended for higher cell viability and longer stability with lyophilization (Morgan et al., 2006; Smith et al., 2008).
Both cryopreservation and lyophilization have advantages and disadvantages, and the response of preservation varies by species. Even different strains of the same species may respond differently to the same preservation method. The viability and longevity of microorganisms under preservation depends on some critical factors: (1) composition of the suspension and rehydration medium, (2) type of cryoprotectant used, (3) rate of cooling and thawing, (4) growth stage of the culture, (5) cell size and type, lipid content, water content, and initial density of cells (De Kievit et al., 2001; Hubalek, 2003; Ren et al., 2004; Morgan et al., 2006; Smith et al., 2008; Chian, 2010)
Reff -
Which of the following is preferable method for the long term storage of Microoraganisms?
The best method for preserving and storing most microorganisms and cells is freezing in the vapor phase of liquid nitrogen (-130 °C and lower). This method is more expensive than freeze drying, but it has a better survival and recovery rate.
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Which of the following is preferable method for the long term storage of Microoraganisms?
- Storage under liquid nitrogen
- Lyophilization
Support your answer with five well-versed statements.