BIO302 Assignment 1 Solution and Discussion

[zareen]

Assignment No. 1
Molecular Biology
Fall 2019

 Total Marks: 10
 Due date: 13/11/2019

Instructions:
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Assignment topic
a) What is bacterial transformation?
b) Write down the steps of bacterial transformation
c) What are the Applications of transformation?
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---------------------------------------------GOOD LUCK---------------------------------------------

[zareen]

@zareen said in BIO302 Assignment 1 Solution and Discussion:

What are the Applications of transformation

When is transformation used?

• To make multiple copies of DNA, called DNA cloning.
• To make large amounts of specific human proteins, for example, human insulin, which can be used to treat people with Type I diabetes.
• To genetically modify a bacterium or other cell.

Source

[zareen]

@zareen said in BIO302 Assignment 1 Solution and Discussion:

Write down the steps of bacterial transformation

Key steps in the process of bacterial transformation: (1) competent cell preparation, (2) transformation of cells, (3) cell recovery, and (4) cell plating.
Source

[zareen]

@zareen said in BIO302 Assignment 1 Solution and Discussion:

What is bacterial transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. … Sometimes the exogenous genetic material may co-exist as a plasmid with chromosomal DNA.