BIO302 Assignment 1 Solution and Discussion
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Assignment No. 1
Molecular Biology
Fall 2019Total Marks: 10 Due date: 13/11/2019Instructions:
• Make sure that you upload the solution file before due date. No assignment will be acceptable through e-mail after the due date.
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Assignment topic
a) What is bacterial transformation?
b) Write down the steps of bacterial transformation
c) What are the Applications of transformation?
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@zareen said in BIO302 Assignment 1 Solution and Discussion:
What are the Applications of transformation
When is transformation used?
- To make multiple copies of DNA, called DNA cloning.
- To make large amounts of specific human proteins, for example, human insulin, which can be used to treat people with Type I diabetes.
- To genetically modify a bacterium or other cell.
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@zareen said in BIO302 Assignment 1 Solution and Discussion:
Write down the steps of bacterial transformation
Key steps in the process of bacterial transformation: (1) competent cell preparation, (2) transformation of cells, (3) cell recovery, and (4) cell plating.
Source
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@zareen said in BIO302 Assignment 1 Solution and Discussion:
What is bacterial transformation
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. … Sometimes the exogenous genetic material may co-exist as a plasmid with chromosomal DNA.
