ZOO630 Assignment 2 Solution and Discussion
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Assignment No. 2
ZOO630 – Research methods in Biology
Fall 2019Total Marks: 10
Due date: 24-01-2020
Instructions:
• Make sure that you upload the solution file before due date. No assignment will be accepted through e-mail after the due date.
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Solution guidelines
• To solve this assignment, you should have good command over lectures 68-105.
• This is not a group assignment, it is an individual assignment so be careful and avoid copying others’ work
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• Give the answer according to question only and avoid irrelevant details.Please note that your assignment will not be graded if:
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• Cheating or copying of assignment is strictly prohibited. The cheated or copied assignment will be marked ‘Zero’.Question No. 1
Discuss factors affecting agarose gel electrophoresis. (10)---------------------------------------------------GOOD LUCK----------------------------------------------
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Separation of nucleic acids by agarose gel electrophoresis works by harnessing the … Agarose gels are cheap and can be used to examine RNA or DNA stability. … precipitate out of solution when stored at higher concentrations (10× solution, … There are three factors that affect migration rate through a gel: size of the DNA, …
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@zareen said in ZOO630 Assignment 2 Solution and Discussion:
Discuss factors affecting agarose gel electrophoresis. (10)
A number of factors can affect the migration of nucleic acids: the dimension of the gel pores (gel concentration), size of DNA being electrophoresed, the voltage used, the ionic strength of the buffer, and the concentration of intercalating dye such as ethidium bromide if used during electrophoresis.
