ZOO630 Assignment 2 Solution and Discussion

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Assignment No. 2
ZOO630 – Research methods in Biology
Fall 2019

Total Marks: 10
Due date: 24-01-2020
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• To solve this assignment, you should have good command over lectures 68-105.
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Question No. 1
Discuss factors affecting agarose gel electrophoresis. (10)

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[zareen]

Separation of nucleic acids by agarose gel electrophoresis works by harnessing the … Agarose gels are cheap and can be used to examine RNA or DNA stability. … precipitate out of solution when stored at higher concentrations (10× solution, … There are three factors that affect migration rate through a gel: size of the DNA, …

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[zareen]

@zareen said in ZOO630 Assignment 2 Solution and Discussion:

Discuss factors affecting agarose gel electrophoresis. (10)

A number of factors can affect the migration of nucleic acids: the dimension of the gel pores (gel concentration), size of DNA being electrophoresed, the voltage used, the ionic strength of the buffer, and the concentration of intercalating dye such as ethidium bromide if used during electrophoresis.

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