ZOO630 Assignment 2 Solution and Discussion


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    Assignment No. 2
    ZOO630 – Research methods in Biology
    Fall 2019

    Total Marks: 10
    Due date: 24-01-2020
    Instructions:
    • Make sure that you upload the solution file before due date. No assignment will be accepted through e-mail after the due date.
    Formatting guidelines
    • Use the font style “Times New Roman” and font size “12”.
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    Solution guidelines
    • To solve this assignment, you should have good command over lectures 68-105.
    • This is not a group assignment, it is an individual assignment so be careful and avoid copying others’ work
    • Plagiarism of assignment file (MS Word) will be checked. There will be negative marking in case of plagiarism detected.
    • Give the answer according to question only and avoid irrelevant details.

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    Question No. 1
    Discuss factors affecting agarose gel electrophoresis. (10)

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  • Cyberian's Gold

    Separation of nucleic acids by agarose gel electrophoresis works by harnessing the … Agarose gels are cheap and can be used to examine RNA or DNA stability. … precipitate out of solution when stored at higher concentrations (10× solution, … There are three factors that affect migration rate through a gel: size of the DNA, …

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  • Cyberian's Gold

    @zareen said in ZOO630 Assignment 2 Solution and Discussion:

    Discuss factors affecting agarose gel electrophoresis. (10)

    A number of factors can affect the migration of nucleic acids: the dimension of the gel pores (gel concentration), size of DNA being electrophoresed, the voltage used, the ionic strength of the buffer, and the concentration of intercalating dye such as ethidium bromide if used during electrophoresis.

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