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BIO601 – Proteomics
Assignment No. 1
Fall 2019
Due Date: 14 Nov 2019
Audio Introduction:
“Please prepare an audio introduction file through any available device. Preferably Sound recorder of the Windows”
General instructions
• Total time of audio introduction will be 5 minutes.
o Course name and code (30 sec) (Very Important)
o Your Introduction (2 minutes)
o Your academic Background (1 minute)
o Views about course (1 minute)
o Expectations about the course ( 30 sec)
• This assignment carries 5 marks.
• “Audio Introduction file” should be uploaded on LMS not later than
14 Nov 2019DO NOT send the assignment thorough email. Assignments submitted through email will result in no marks.
Instructions for audio recording through Sound recorder of the windows
• Open Windows Search
• Write “Sound recorder” in the search bar and click enter
• Select the program “Sound recorder” Open it and start recording.
• Save the file and send it through the LMS.Outcomes:
This assignment will help you to develop presentation skills and strengthen you to present yourself in front of audience. -
Total Marks 5
Starting Date Monday, January 27, 2020
Closing Date Tuesday, January 28, 2020
Status Open
Question Title Discuss Microarray and applications of microarray.
Question DescriptionDiscussion Question:
Discuss Microarray and applications of microarray.
Important Instructions:
Your discussion must be based on logical facts.
Your comments on the topic should not exceed 200-250 words.
The GDB will remain open for 2 working days/ 48hours.
Do not copy or exchange your answer with other students. Two identical / copied comments will be marked Zero (0) and may damage your grade in the course.
Obnoxious or ignoble answer should be strictly avoided.
Questions / queries related to the content of the GDB, which may be posted by the students on MDB or via e-mail, will not be replied till the due date of GDB is over.
For Detailed Instructions please see the GDB Announcement.
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Discussion Question:
Which method is most suitable to identify an unknown protein on the basis of its molecular mass?
Important Instructions:
Your discussion must be based on logical facts.
Your comments on the topic should not exceed 200-250 words.
Support your answer with 5 well-versed statements.
The GDB will remain open for 2 working days/ 48hours.
Do not copy or exchange your answer with other students. Two identical / copied comments will be marked Zero (0) and may damage your grade in the course.
Obnoxious or ignoble answer should be strictly avoided.
Questions / queries related to the content of the GDB, which may be posted by the students on MDB or via e-mail, will not be replied till the due date of GDB is over.
For Detailed Instructions please see the GDB Announcement.
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Assignment No. 2
Total Marks: 10 Due date: 05-08-2019
BIO601 – Proteomics
Spring 2019Instructions:
Make sure that you upload the solution file before due date. No assignment will be accepted through e-mail after the due date.
Formatting guidelines
• Use the font style “Times New Roman” and font size “12”.
• Compose your document in MS-Word, any file created in any format will not be accepted and marked zero.
• Use black and blue font colors only.
Solution guidelines
• To solve this assignment, you should have good command over lectures 60-65.
• This is not a group assignment, it is an individual assignment so be careful and avoid copying others’ work
• Give the answer according to question only and avoid irrelevant details.Please note that your assignment will not be graded if:
• It is submitted after due date
• The file you uploaded does not open
• The file you uploaded is copied from someone else
• It is in some format other than .doc
• Cheating or copying of assignment is strictly prohibited. The cheated or copied assignment will be marked ‘Zero’.Question No. 1
Write down the principle and procedure of western blotting. (10)---------------------------------------------------GOOD LUCK----------------------------------------------
SOLVED BIO601 GDB1 Solution and discussion
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Discussion Question:
Which method is most suitable to identify an unknown protein on the basis of its molecular mass?
Important Instructions:
-
Your discussion must be based on logical facts.
-
Your comments on the topic should not exceed 200-250 words.
-
Support your answer with 5 well-versed statements.
-
The GDB will remain open for 2 working days/ 48hours.
-
Do not copy or exchange your answer with other students. Two identical / copied comments will be marked Zero (0) and may damage your grade in the course.
-
Obnoxious or ignoble answer should be strictly avoided.
-
Questions / queries related to the content of the GDB, which may be posted by the students on MDB or via e-mail, will not be replied till the due date of GDB is over.
For Detailed Instructions please see the GDB Announcement.
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Methods for Protein Analysis
- Protein Separation Methods
- Western Blotting
- Protein Identification Methods
3A. Edman degradation
3B. Mass spectrometry
However, and perhaps remarkably, if as shown above we digest samples of ourprotein with as few as two or three different endoproteases with different
specificities, we can usually use the resulting digestion patterns (again, analyzedby mass spectrometry to provide highly accurate determination of the fragmentmolecular weights) to produce a unique identification of our unknown protein:Again, mass spectrometry is uniquely well-suited for such analyses because itcan yield very accurate determinations of molecular weights from very even verysmall amounts of fragments resulting from the digestion of a particular protein.Techniques have now been developed by which proteins separated in two-dimensional gels can be digested within the gels and then injected directly into amass spectrometer for analysis of the resulting fragments.