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Discus practical applications of HPLC & GC-MS?
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Assignment No. 2
CHE301 – Analytical Chemistry & Instrumentaions
Fall 2019Total Marks: 10
Due date: 16-01-2020
Instructions:
• Make sure that you upload the solution file before due date. No assignment will be accepted through e-mail after the due date.
Formatting guidelines
• Use the font style “Times New Roman” and font size “12”.
• Compose your document in MS-Word, any file created in any other format will not be accepted and assigned zero mark.
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Solution guidelines
• To solve this assignment, you should have good command over lectures 64-74.
• This is not a group assignment, it is an individual assignment so be careful and avoid copying others’ work
• Plagiarism of assignment file (MS Word) will be checked. There will be negative marking in case of plagiarism detected.
• Give the answer according to question only and avoid irrelevant details.Please note that your assignment will not be graded if:
• It is submitted after due date
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• Cheating or copying of assignment is strictly prohibited. The cheated or copied assignment will be marked ‘Zero’.Question No. 1
Discuss Instrumentation & working of HPLC? (10)---------------------------------------------------GOOD LUCK----------------------------------------------
CHE301 GDB 1 Solution and Discussion
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Discus practical applications of HPLC & GC-MS?
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@zareen said in CHE301 GDB 1 Solution and Discussion:
Discus practical applications of HPLC & GC-MS?
Gas chromatography (GC) is used widely in applications involving food analysis. Typical applications pertain to the quantitative and/or qualitative analysis of food composition, natural products, food additives, flavor and aroma components, a variety of transformation products, and contaminants, such as pesticides, fumigants, environmental pollutants, natural toxins, veterinary drugs, and packaging materials. The aim of this article is to give a brief overview of the many uses of GC in food analysis in comparison to high-performance liquid chromatography (HPLC) and to mention state-of-the-art GC techniques used in the major applications. Past and current trends are assessed, and anticipated future trends in GC for food applications are predicted. Among the several new techniques being developed, the authors believe that, in food analysis applications, fast-GC/mass spectrometry (MS) will have the most impact in the next decade. Three approaches to fast-GC/MS include low-pressure GC/MS, GC/time-of-flight (TOF)-MS and GC/supersonic molecular beam (SMB)-MS, which are briefly discussed, and their features are compared.
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